THE SMART SOLUTION FOR
POLYCLONAL ANTIBODY PRODUCTION
POLYCLONAL ANTIBODY PRODUCTION
For over 30 years, Noble Life Sciences has supplied the research community with GLP and non-GLP custom polyclonal antibody services using a variety of animal species including mouse, rat, rabbit, guinea pig, ferret, sheep and goat. Client-specific protocols have been used as well as Noble-developed standard immunization protocols. Peptide conjugation, antibody titer analysis via ELISA, and antibody purification from serum are available. Peptide sequence analysis and synthesis are also available. Further extension of custom polyclonal services includes performing immunogenicity and efficacy studies in rats, mice and rabbits for large and small pharmaceutical companies. Click here for an overview of our custom polyclonal antibodies services.
Standard Protocol
Day 0 | Pre-immunization bleed Primary immunization |
Day 21 | Immunogen boost |
Day 42 | Immunogen boost |
Day 52 | Standard bleed* |
Day 63 | Immunogen boost |
Day 73 | Standard bleed or exsanguination |
Fast Protocol
Day 0 | Pre-immunization bleed Primary immunization |
Day 21 | Immunogen boost |
Day 31 | Standard bleed* |
Day 43 | Immunogen boost |
Day 52 | Standard bleed or exsanguination |
ANTIGEN PREPARATION
Noble can accept antigen for immunization in any form including, but not limited to, in solution, as a precipitate, as a cell suspension, as a band in acrylamide gel, or as a blot on nitrocellulose. Freund’s Complete Adjuvant is used for the initial injection and Freund’s Incomplete Adjuvant is used for subsequent injections. Other adjuvants are available.
Approximate Serum Volume by Species (ml)
Procedure | Rabbit | Guinea Pig, Ferret & Rat | Mouse | Sheep & Goat |
---|---|---|---|---|
Test bleed | 2 | 0.5 | 0.15 | 15 |
Pre-bleed | 4.5 | 1 | 0.15 | 50 |
Standard bleed | 15 | 1.5 | 0.2 | 300 |
Exsanguination | 50 | 6 | 0.3 | 900 |
Plasmapheresis | – | – | – | 1500 |
Minimum Antigen Requirements
Standard protocol (total per animal or 5 mice) | 750 mcg |
Fast protocol (total per animal or 5 mice) | 500 mcg |
Protocol extension (total per animal or 5 mice) | 250 mcg |
ELISA (up to 6 samples) | 20 mcg |
Western blot (up to 6 samples) | 20 mcg |
Options
The following options are available for tailoring your protocol:
- GLP and non-GLP
- Background screening
- ELISA
- Antibody purification: affinity or protein A/G
- Serum filtration
- Western blot
- Peptide sequence prediction,synthesis and congugation
- Monthly extensions
To send us a sample please complete our submission form.

GLP Antibody Production
Our GLP custom polyclonal antibodies are ideal for evaluating the potency, identity, and purity of biologics. Click here for an overview of our custom polyclonal antibodies services.
Proven Expertise
We have experience with a variety of mammalian species used for vaccine and drug development. Our studies range from single digit to hundreds of animals.
Our scientists, with over 15 years of GLP experience, provide all aspects of the GLP process from design to regulatory support of your IND, IDE or PMA FDA submission.