SERVICES

Digital PCR (dPCR)

Elevate your studies with our comprehensive dPCR services, providing unparalleled accuracy and valuable insights for your nucleic acid quantification needs.

As your preclinical contract research organization partner, we provide Digital PCR (dPCR) services for various applications in life sciences research. With our expertise, cutting-edge technology, and GLP labs, we offer comprehensive solutions to support your digital PCR needs.

Value of Digital PCR (dPCR)

Our dPCR services provide several key benefits for your preclinical research:

  • Enhanced Sensitivity: dPCR offers increased sensitivity and precision, enabling the detection and quantification of rare targets or subtle changes in gene expression.
  • Absolute Quantification: dPCR allows for absolute quantification of nucleic acids, eliminating the need for external references or a standard curve and thus providing accurate and reliable results.
  • Improved Precision: The partitioning of samples in digital PCR reduces the impact of PCR inhibitors and enhances the precision of measurements, ensuring more reliable data.
  • High Throughput: Our dPCR services are designed to handle high-throughput projects, allowing for the simultaneous analysis of multiple samples, saving you time and resources.
  • Flexibility: We offer customization options to meet your specific research requirements, including assay design, target selection, and data analysis.

Our Capabilities

  • Gene Expression Analysis: Our dPCR services enable precise measurement of gene expression levels, offering insights into molecular pathways, disease progression, and therapeutic responses.
  • Cell and Gene Therapy Applications: dPCR can confidently detect and quantify the amount of lentivirus product produced, ensuring accurate dosing. We can also test fr the presence of residual DNA in sample preparations to ensure purity and safety.
  • Rare Mutation Detection: We can accurately detect and quantify rare genetic mutations, aiding in early diagnosis, prognosis, and monitoring of diseases.
  • Copy Number Variation Analysis: Our expertise in dPCR allows for the detection and quantification of copy number variations, helping to understand genetic diversity and disease associations.
  • Pathogen Detection: We offer dPCR-based assays for the sensitive and specific detection of pathogens, including viruses, bacteria, and fungi, enabling efficient surveillance and monitoring.
  • Environmental Testing: Our dPCR services can be applied to environmental testing applications, such as monitoring water quality, detecting genetically modified organisms, or assessing biodiversity.
  • Residual DNA Quantification: dPCR can be used to quantify residual DNA in the quality control process for manufactured products such as monoclonal antibodies, plasmid DNA, and proteins/peptides, thus ensuring compliance with regulatory guidelines and assessing product safety and purity.
  • miRNA Quantification: dPCR’s high sensitivity and specificity can not only reliably identify and quantify miRNA but discriminate closely related and/or mature miRNA from precursors. Some miRNAs represent biomarkers that can indicate certain types of cancers.
  • Biodistribution Analysis: dPCR enables precise quantification of therapeutic DNA or RNA molecules in various tissues and organs, offering valuable insights into their safety, efficacy, and distribution patterns to help researchers optimize treatment strategies for improved clinical outcomes.
dPCR capabilities
Noble Life Sciences

QIAcuity QIAGEN Machine

Our state-of-the-art dPCR platform includes the QIAcuity instrument from QIAGEN. This advanced machine offers precise and reliable digital PCR capabilities and exceptional data quality. With the QIAcuity system, we ensure accurate and reproducible results for your digital PCR studies.

 

  • High-throughput: 4-plate capacity allows quantification of up to 768 dPCR samples (256 for triplicate reactions) in a single workday
  • Multiplexing capabilities: Up to 5 color channels for multiplex quantification of up to 5 target DNA and/or RNA molecules in a given assay
  • Flexibility: Distinct nanoplate configurations with flexible sample formats that accommodate a wide range of throughput and sensitivity requirements

Read More About Noble’s dPCR Assay Services

Discover the science behind our dPCR competencies.

Frequently Asked Questions (FAQs) About Digital PCR (dPCR) Services

What is digital PCR?

Digital PCR (dPCR) is a nucleic acid amplification technique used to precisely quantify and analyze target DNA or RNA molecules. It involves partitioning the sample into multiple individual reactions, allowing for the absolute quantification of the target molecules without the need for a standard curve.

What is dPCR?

dPCR stands for digital PCR, which is a powerful technique that enables the absolute quantification of target nucleic acids. It provides improved sensitivity and precision compared to traditional quantitative PCR (qPCR) methods.

What is digital PCR used for?

Digital PCR has diverse applications in various fields, including:

  • Quantification of gene expression levels.
  • Cell and gene therapy applications
  • Detection and quantification of rare genetic mutations
  • Copy number variation analysis
  • miRNA qualification
  • Pathogen detection in clinical diagnostics
  • Environmental testing, such as monitoring water quality or assessing biodiversity
  • Residual DNA QC test of manufactured products
  • In vivo biodistribution analysis of cell and gene therapeutics in safety studies

How does digital PCR work?

Digital PCR works by partitioning a sample into numerous individual reactions, each containing a target molecule, a few target molecules, or none at all. These reactions undergo PCR amplification, where the target molecules are replicated. After amplification, the reactions are assessed to determine the number of positive and negative reactions. By applying Poisson distribution statistics, the absolute quantity of target molecules in the original sample can be calculated based on the ratio of positive to negative reactions. This method allows for precise quantification of target molecules, even at low concentrations, making digital PCR a powerful tool for various applications, including the quantification of residual DNA, gene expression analysis, and detection of rare genetic variants.

What is digital droplet PCR?

Digital droplet PCR (ddPCR) is a specific type of digital PCR that utilizes water-oil emulsion droplets to partition the sample. The target molecules are randomly distributed into the droplets, and each droplet serves as a discrete PCR reaction. This method offers high sensitivity and precision for target quantification.

What is the difference between dPCR vs qPCR?

The main difference between digital PCR (dPCR) and quantitative PCR (qPCR) lies in the quantification approach. While qPCR provides relative quantification by comparing the target amplification to a reference, dPCR offers absolute quantification by directly counting the target molecules. dPCR is advantageous for its improved sensitivity, precision, and the ability to detect rare targets accurately.

Contact us about our dPCR services

Discover the power of tailored dPCR services by reaching out to us today, and let us customize our offerings to meet your specific research needs.

Interested in dPCR assays for Structural Variants and Genome Stability? 

Noble Life Sciences and KromaTiD created the ultimate assay for genome stability, DNA repair, and mutagenic activity.