As your preclinical contract research organization partner, we provide Digital PCR (dPCR) services for various applications in life sciences research. With our expertise, cutting-edge technology, and GLP labs, we offer comprehensive solutions to support your digital PCR needs.
Value of Digital PCR (dPCR)
Our dPCR services provide several key benefits for your research:
- Enhanced Sensitivity: Digital PCR offers increased sensitivity and precision, enabling the detection and quantification of rare targets or subtle changes in gene expression.
- Absolute Quantification: dPCR allows for absolute quantification of nucleic acids, eliminating the need for external references and providing accurate and reliable results.
- Improved Precision: The partitioning of samples in digital PCR reduces the impact of PCR inhibitors and enhances the precision of measurements, ensuring more reliable data.
- High Throughput: Our dPCR services are designed to handle high-throughput projects, allowing for the simultaneous analysis of multiple samples, saving time and resources.
- Flexibility: We offer customization options to meet your specific research requirements, including assay design, target selection, and data analysis.
Our Capabilities
- Gene Expression Analysis: Our dPCR services enable precise measurement of gene expression levels, offering insights into molecular pathways, disease progression, and therapeutic responses.
- Rare Mutation Detection: We can accurately detect and quantify rare genetic mutations, aiding in early diagnosis, prognosis, and monitoring of diseases.
- Copy Number Variation Analysis: Our expertise in dPCR allows for the detection and quantification of copy number variations, helping to understand genetic diversity and disease associations.
- Pathogen Detection: We offer dPCR-based assays for the sensitive and specific detection of pathogens, including viruses, bacteria, and fungi, enabling efficient surveillance and monitoring.
- Environmental Testing: Our dPCR services can be applied to environmental testing, such as monitoring water quality, detecting genetically modified organisms, or assessing biodiversity.
- Residual DNA Quantification: Digital PCR is a sensitive method used for residual DNA quantification in quality control of manufactured products such as monoclonal antibodies, plasmid DNA, and proteins/peptides. It partitions the sample into numerous individual PCR reactions to detect and quantify trace amounts of residual DNA. By analyzing the distribution of positive and negative reactions, the absolute quantity of residual DNA can be determined with high accuracy, ensuring compliance with regulatory guidelines and assessing product safety and purity.
- Biodistribution Analysis: Digital PCR plays a crucial role in biodistribution studies of cell and gene therapies. It enables precise quantification of therapeutic DNA or RNA molecules in various tissues and organs, offering valuable insights into their distribution patterns. By providing highly sensitive and accurate measurements of target molecules, digital PCR helps researchers assess the safety and efficacy of these therapies, monitor their biodistribution profiles, and optimize treatment strategies for improved clinical outcomes.
QIAcuity QIAGEN Machine
Our state-of-the-art dPCR platform includes the QIAcuity instrument from QIAGEN. This advanced machine offers precise and reliable digital PCR capabilities, featuring high sample throughput and exceptional data quality. With the QIAcuity system, we ensure accurate and reproducible results for your digital PCR studies.
Learn More About Our dPCR Capabilities
Discover the science behind our dPCR competencies.
Frequently Asked Questions (FAQs) About Digital PCR (dPCR) Services
What is digital PCR?
What is dPCR?
What is digital PCR used for?
Digital PCR has diverse applications in various fields, including:
- Quantification of gene expression levels.
- Detection and quantification of rare genetic mutations.
- Copy number variation analysis.
- Pathogen detection in clinical diagnostics.
- Environmental testing, such as monitoring water quality or assessing biodiversity.
- Residual DNA QC test of manufactured products.
- In vivo biodistribution analysis of cell and gene therapeutics in safety studies.
How does digital PCR work?
Digital PCR works by partitioning a sample into numerous individual reactions, each containing a target molecule, a few target molecules, or none at all. These reactions undergo PCR amplification, where the target molecules are replicated. After amplification, the reactions are assessed to determine the number of positive and negative reactions. By applying Poisson distribution statistics, the absolute quantity of target molecules in the original sample can be calculated based on the ratio of positive to negative reactions. This method allows for precise quantification of target molecules, even at low concentrations, making digital PCR a powerful tool for various applications, including the quantification of residual DNA, gene expression analysis, and detection of rare genetic variants.
What is digital droplet PCR?
What is the difference between dPCR vs qPCR?
Learn more about this service
Discover the power of tailored dPCR services by reaching out to us today, and let us customize our offerings to meet your specific research needs.
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Founded by accomplished industry scientists
Noble offers everything needed to run a successful preclinical study:
24,000 square feet fully-equipped facility
AAALAC accredited small and large animal facility
Full continuum of GLP and non-GLP services
Spectrum of in vitro and in vivo services