Animals are infected intranasally with RSV strain A2. The time course of the infection is followed by determining viral titers in lung homogenates or bronchoalveolar lavage fluids (BALF).
The efficacy of test agents in animals challenged with the virus is determined at peak viral titers.
Viral titers are determined via plaque assay or qPCR. H&E stained sections of lung tissue can be prepared for evaluation of tissue histology.