THE SMART SOLUTION FOR
PRECLINICAL DEVELOPMENT

NEUTROPENIC THIGH INFECTION MODEL

 

SERVICE DESCRIPTION

The neutropenic mouse thigh model of infection is a platform for the in vivo study of antibacterial agents under conditions optimal for efficacy, i.e., neutropenia.  Because the pharmacology of antimicrobial agents is affected by the presence of neutrophils, animals are rendered neutropenic with the immunosuppressive agent, cyclophosphamide.

APPLICATIONS

  • Comparison of antimicrobial agents and dosing regimens
  • Determination of PK/PD indices related to efficacy (and prevention of emergence of resistance) of an antimicrobial agent
  • Determination of the time course of antimicrobial activity – concentration or time dependent; presence/absence of persistent effects
  • Identification of factors that affect the magnitude of PK/PD indices – CFU changes (short course of therapy) vs survival (long course of therapy)
  • Determination of dose, dosing interval, and susceptibility breakpoints
  • Testing/benchmarking antimicrobial drugs; determination of therapeutic equivalence of generic products
Click Here for Technical Note – Mouse Thigh Infection

METHODOLOGY

Animals made neutropenic and infected with bacterial strain of interest on the thigh muscle. The efficacy of test agents in animals challenged with bacteria is determined by measuring bacterial load in infected tissues.

OPTIONS

  • Animal Species: Mice, rats, guinea pigs
  • Drug Administration Route: Intranasal, intravenous, intramuscular, continuous infusion

  • Reference Substance & Control: Vancomycin (or other reference antibiotic); vehicle

  • Length/Endpoint: Twenty four to five days post infection

  • Assays & Measurements:  bacterial load in the muscle tissue,  immunohistochemistry, tissue pathology, cytokine, chemokine levels

APPLICATIONS

  • Testing/benchmarking antimicrobial drugs
  • Determination of dose, dosing interval and in vivo susceptibility break points
  • Determination of PK/PD indices related to efficacy
Click Here for Technical Note – Guinea Pig Thigh Infection

Proven Expertise

We have experience with a variety of mammalian species used for vaccine and drug development. Our studies range from single digit to hundreds of animals.

Our scientists, with over 15 years of GLP experience, provide all aspects of the GLP process from design to regulatory support of your IND, IDE or PMA FDA submission.

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