Mice are euthanized and their ceca removed. Cecal contents are extracted, diluted with cold 5% dextrose and passed through an 18-gauge needle to break up solids, and stored in aliquots at -80oC.
For injection, the cecal slurry is thawed rapidly at 37oC and then equilibrated to room temperature. Groups of mice receive intraperitoneal (IP) injections at doses ranging from 0.5 gm/kg to 4 gm/kg. Animals are monitored and deaths recorded.
If preferred, the model can be run using a modified Schwartzman reaction where a priming dose of lipopolysaccharide is given to the mice to sensitize them prior to administration of the cecal slurry.