Noble Life Sciences is a trusted contract research organization and provider of qPCR (quantitative polymerase chain reaction) services, offering comprehensive solutions to meet the diverse needs of life sciences research. With our expertise and cutting-edge technology, including the advanced QuantStudio 6 system, we deliver accurate and reliable results to advance your scientific endeavors.
Value of qPCR Services
- Accurate Quantification: Our qPCR services ensure precise quantification of target nucleic acids, providing valuable insights into gene expression levels, genetic variations, copy number quantification, and biomarker analysis.
- Sensitivity and Specificity: Our advanced qPCR techniques ensure high sensitivity and specificity, allowing for the detection of low-abundance targets and discrimination of closely related sequences.
- Rapid Turnaround: We offer efficient turnaround times for our qPCR services, allowing you to obtain reliable data quickly, facilitating timely decision-making in your research projects.
- Customized Approach: We tailor our qPCR services to your specific requirements, including qPCR assay development, target selection, and data analysis, ensuring the most relevant and actionable results for your study.
Our Capabilities
- Gene Expression Analysis: Our qPCR services enable accurate measurement of gene expression levels, providing insights into molecular pathways, disease mechanisms, and therapeutic responses.
- SNP Genotyping: We specialize in qPCR-based SNP genotyping, allowing for the detection of genetic variations associated with diseases, drug response, and population studies.
- MicroRNA Analysis: We offer qPCR-based assays for microRNA analysis, facilitating the investigation of regulatory roles in gene expression and their implications in diseases.
- Pathogen Detection: Our qPCR services include the detection and quantification of pathogens, such as viruses, bacteria, and fungi, aiding in clinical diagnostics and surveillance.
- Biomarker Validation: We provide qPCR services for biomarker validation, enabling the assessment of potential diagnostic, prognostic, and predictive markers for various diseases.
- Biodistribution Analysis: We offer comprehensive qPCR services for biodistribution studies, specifically tailored to evaluate the in vivo safety profile of cell and gene therapeutics. Our specialized qPCR services enable precise detection and quantification of therapeutic molecules within various tissues and organs. By employing state-of-the-art techniques and rigorous protocols, we ensure accurate analysis of the distribution and persistence of therapeutic agents throughout the body.
qPCR Standards
Thermo Scientific QuantStudio 6 Flex qPCR System
Frequently Asked Questions (FAQs) About qPCR
What is qPCR?
How to normalize qPCR data?
What is the difference between RT-PCR and PCR?
How does qPCR work?
Why is PCR a valuable technique?
How to do qPCR?
Is RT-PCR the same as PCR?
How does qPCR measure gene expression?
To determine the quantity of the target DNA, a standard curve is employed. The standard curve is generated by running qPCR reactions with known concentrations of a reference DNA sample. The cycle threshold (Ct) values obtained from the amplification of the reference DNA at different concentrations are plotted against the logarithm of the DNA concentration. By comparing the Ct value of the unknown samples with the standard curve, the quantity of the target DNA in the unknown samples can be estimated accurately.
How does qPCR measure the quantity of the target DNA?
qPCR measures gene expression by amplifying the target RNA (converted to cDNA) using gene-specific primers. The amplified cDNA is then quantified in real-time, providing information about the initial RNA quantity. Higher gene expression leads to earlier amplification signals (a lower threshold cycle number Ct).
Learn more about this service
Discover the power of tailored dPCR services by reaching out to us today, and let us customize our offerings to meet your specific research needs.