qPCR with QuantStudio 6

Elevate your studies with our comprehensive qPCR services, powered by the advanced QuantStudio 6 system. Experience unparalleled accuracy and valuable insights for your nucleic acid quantification needs.

Noble Life Sciences is a trusted contract research organization and provider of qPCR (quantitative polymerase chain reaction) services, offering comprehensive solutions to meet the diverse needs of life sciences research. With our expertise and cutting-edge technology, including the advanced QuantStudio 6 system, we deliver accurate and reliable results to advance your scientific endeavors.

Value of qPCR Services

Our qPCR services offer several key benefits for your research:
  • Accurate Quantification: Our qPCR services ensure precise quantification of target nucleic acids, providing valuable insights into gene expression levels, genetic variations, copy number quantification, and biomarker analysis.
  • Sensitivity and Specificity: Our advanced qPCR techniques ensure high sensitivity and specificity, allowing for the detection of low-abundance targets and discrimination of closely related sequences.
  • Rapid Turnaround: We offer efficient turnaround times for our qPCR services, allowing you to obtain reliable data quickly, facilitating timely decision-making in your research projects.
  • Customized Approach: We tailor our qPCR services to your specific requirements, including qPCR assay development, target selection, and data analysis, ensuring the most relevant and actionable results for your study.
Noble Life Sciences

Our Capabilities

  • Gene Expression Analysis: Our qPCR services enable accurate measurement of gene expression levels, providing insights into molecular pathways, disease mechanisms, and therapeutic responses.
  • SNP Genotyping: We specialize in qPCR-based SNP genotyping, allowing for the detection of genetic variations associated with diseases, drug response, and population studies.
  • MicroRNA Analysis: We offer qPCR-based assays for microRNA analysis, facilitating the investigation of regulatory roles in gene expression and their implications in diseases.
  • Pathogen Detection: Our qPCR services include the detection and quantification of pathogens, such as viruses, bacteria, and fungi, aiding in clinical diagnostics and surveillance.
  • Biomarker Validation: We provide qPCR services for biomarker validation, enabling the assessment of potential diagnostic, prognostic, and predictive markers for various diseases.
  • Biodistribution Analysis: We offer comprehensive qPCR services for biodistribution studies, specifically tailored to evaluate the in vivo safety profile of cell and gene therapeutics. Our specialized qPCR services enable precise detection and quantification of therapeutic molecules within various tissues and organs. By employing state-of-the-art techniques and rigorous protocols, we ensure accurate analysis of the distribution and persistence of therapeutic agents throughout the body.
qPCR Noble Life Sciences capabilities

qPCR Standards

We adhere to rigorous qPCR standards to ensure the accuracy and reliability of our results. We follow established guidelines and best practices, including the use of appropriate controls, quality assurance measures, and standard operating procedures (SOPs), to maintain the highest standards of quality in our qPCR services.

Thermo Scientific QuantStudio 6 Flex qPCR System

We utilize the Thermo Scientific QuantStudio 6 Flex qPCR System, a state-of-the-art instrument known for its versatility, accuracy, and sensitivity. This system offers a wide dynamic range, multiple reaction formats, and intuitive software for data analysis, ensuring the highest quality results for your qPCR experiments.
Noble Life Sciences

Frequently Asked Questions (FAQs) About qPCR

What is qPCR?

qPCR, or quantitative polymerase chain reaction, is a technique used to measure the amount of DNA or RNA in a sample. It provides quantitative data by amplifying the target nucleic acid and monitoring the fluorescence signal in real-time.

How to normalize qPCR data?

Normalization of qPCR data is essential to account for variations in sample quantity and quality. Common methods include using reference genes (housekeeping genes) or employing normalization algorithms based on total RNA or genomic DNA content.

What is the difference between RT-PCR and PCR?

RT-PCR (Reverse Transcription PCR) is a variation of PCR that involves converting RNA into complementary DNA (cDNA) before amplification. PCR, on the other hand, amplifies DNA directly. RT-PCR is used to study gene expression levels, while PCR is employed for DNA amplification.

How does qPCR work?

qPCR works by using specific primers to amplify the target DNA or cDNA in cycles. The amplification is monitored in real-time using fluorescent probes or DNA-binding dyes. The increase in fluorescence is directly proportional to the amount of amplified target, allowing quantification.

Why is PCR a valuable technique?

PCR is a valuable technique because it allows the amplification of specific DNA sequences, even from minute amounts of starting material. It has revolutionized molecular biology, enabling applications such as gene cloning, sequencing, diagnostics, and gene expression analysis.

How to do qPCR?

Performing qPCR involves several steps: designing specific primers and probes, preparing the reaction mix, adding the template DNA or cDNA, running the PCR program, and analyzing the data using appropriate software. It requires precise laboratory techniques and well-optimized protocols.

Is RT-PCR the same as PCR?

RT-PCR (Reverse Transcription PCR) is a specific type of PCR that involves the conversion of RNA into complementary DNA (cDNA) before amplification. Regular PCR refers to the amplification of DNA directly. While similar in principle, they differ in the starting material and purpose.

How does qPCR measure gene expression?

To determine the quantity of the target DNA, a standard curve is employed. The standard curve is generated by running qPCR reactions with known concentrations of a reference DNA sample. The cycle threshold (Ct) values obtained from the amplification of the reference DNA at different concentrations are plotted against the logarithm of the DNA concentration. By comparing the Ct value of the unknown samples with the standard curve, the quantity of the target DNA in the unknown samples can be estimated accurately.

How does qPCR measure the quantity of the target DNA?

qPCR measures gene expression by amplifying the target RNA (converted to cDNA) using gene-specific primers. The amplified cDNA is then quantified in real-time, providing information about the initial RNA quantity. Higher gene expression leads to earlier amplification signals (a lower threshold cycle number Ct).

Learn more about this service

Discover the power of tailored dPCR services by reaching out to us today, and let us customize our offerings to meet your specific research needs.

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